The DESY Nanolaboratory (DESY NanoLab) is a facility providing access to advanced nano-characterization, nano-structuring and nano-synthesis techniques which are complementary to the advanced X-ray techniques available at DESY’s lightsources. The DESY NanoLab consists of several strong pillars in nanoscience research: microscopy, nano-structuring, spectroscopy, nanoparticle synthesis, in-situ and operando X-ray diffraction, electrochemistry, and magnetism. A laser lab is planned for the preparation of ultrafast pump-probe experiments in cooperation with Leibniz-Institut für Kristallzüchtung (IKZ) to complement the NanoLab science fields. The methods available are complementary to the photon-based techniques at the DESY X-ray facilities PETRA III and FLASH and the European XFEL. Access to the DESY NanoLab for external users is granted via regular PETRA III or FLASH proposals and European access programmes, such as NFFA Europe. Industrial users can access via DESY Innovation and Technology Transfer (ITT).