Shadowgraph is an optical method that reveals non-uniformities in transparent media like air, water, or glass. It is related to, but simpler than, the schlieren and schlieren photography methods that perform a similar function. Shadowgraph is a type of flow visualisation.
In principle, a difference in temperature, a different gas, …

Two-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up to about one millimeter in thickness. Unlike traditional fluorescence microscopy, where the excitation wavelength is shorter than the emission wavelength, two-photon excitation requires simultaneous excitation by two photons …

Multispectral imaging captures image data within specific wavelength ranges across the electromagnetic spectrum. The wavelengths may be separated by filters or detected with the use of instruments that are sensitive to particular wavelengths, including light from frequencies beyond the visible light range (i.e. infrared and ultraviolet). It can allow extraction …

NanoSIMS (nanoscale secondary ion mass spectrometry) is an analytical instrument manufactured by CAMECA which operates on the principle of secondary ion mass spectrometry. The NanoSIMS is used to acquire nanoscale resolution measurements of the elemental and isotopic composition of a sample. The NanoSIMS is able to create nanoscale maps of …

Nanotomography, much like its related modalities tomography and microtomography, uses x-rays to create cross-sections from a 3D-object that later can be used to recreate a virtual model without destroying the original model, applying Nondestructive testing. The term nano is used to indicate that the pixel sizes of the cross-sections are …

In microscopy, negative staining is an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid. In this technique, the background is stained, leaving the actual specimen untouched, and thus visible. This contrasts with positive staining, in which the actual specimen is stained.

Neutron tomography is a form of computed tomography involving the production of three-dimensional images by the detection of the absorbance of neutrons produced by a neutron source. It creates a three-dimensional image of an object by combining multiple planar images with a known separation. It has a resolution of down …

Neutron imaging is the process of making an image with neutrons. The resulting image is based on the neutron attenuation properties of the imaged object. The resulting images have much in common with industrial X-ray images, but since the image is based on neutron attenuating properties instead of X-ray attenuation …

Nuclear magnetic resonance spectroscopy, most commonly known as NMR spectroscopy or magnetic resonance spectroscopy (MRS), is a spectroscopic technique based on re-orientation of atomic nuclei with non-zero nuclear spins in an external magnetic field. This re-orientation occurs with absorption of electromagnetic radiation in the radio frequency region from roughly 4 …

Electron holography is holography with electron matter waves. It was invented by Dennis Gabor in 1948 when he tried to improve image resolution in electron microscope. The first attempts to perform holography with electron waves were made by Haine and Mulvey in 1952; they recorded holograms of zinc oxide crystals …

The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in …

Phase-contrast microscopy (PCM) is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are invisible, but become visible when shown as brightness variations. When light waves travel through a medium other than a vacuum, interaction …