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Negative staining

Negative stain

In microscopy, negative staining is an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid. In this technique, the background is stained, leaving the actual specimen untouched, and thus visible. This contrasts with positive staining, in which the actual specimen is stained.

Read more about 'Negative stain' at: Wikipedia

Wikipedia contributors. "Negative stain." Wikipedia, The Free Encyclopedia. Wikipedia, The Free Encyclopedia, Sept. 2, 2024.

Negative staining in Helmholtz Imaging CONNECT:

Expert

Timo Christian

Deutsches Elektronen-Synchrotron - Hamburg

Project Manager for the Helmholtz Transfer Academy "Future Innovators". Interested in anything that can solve problems. Trying to unlock the potential of scientific development to also solve short-term problems of the real-world.

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Facility

Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons (ER-C)

The Ernst Ruska-Centre (ER-C) for Microscopy and Spectroscopy with Electrons is a national user facility open to universities, research institutions and research laboratories in industry. The ER-C is run conjointly by Forschungszentrum Jülich GmbH and …

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Center

Deutsches Elektronen-Synchrotron (DESY)

DESY is one of the world’s leading accelerator centres. Researchers use the large-scale facilities at DESY to explore the microcosm in all its variety – from the interactions of tiny elementary particles and the behaviour …

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Center

Forschungszentrum Jülich (FZJ)

Forschungszentrum Jülich is a large-scale national research facility in the Helmholtz Association of German Research Centres. By conducting research on the global challenges currently facing society, we contribute to the welfare of mankind and responsible …

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Instrument

TFS Talos L120C EM (Talos 120)

The Talos L120C Electron microscope is a robust tool routinely used for initial screening of a biological sample’s purity and homogeneity. The main application at the ER-C is negative staining – a heavy metal embedding …

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Lab

Sachse Lab (Sachse Lab)

We use an integrated structural electron microscopy approach to study the biological structures of membrane-associated protein complexes. Our main methods of investigation are single-particle electron cryo-microscopy (cryo-EM) as well electron cryo-tomography (cryo-ET) that we are …

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