High-energy X-rays or HEX-rays are very hard X-rays, with typical energies of 80–1000 keV (1 MeV), about one order of magnitude higher than conventional X-rays used for X-ray crystallography (and well into gamma-ray energies over 120 keV). They are produced at modern synchrotron radiation sources such as the Cornell High …

High-resolution transmission electron microscopy is an imaging mode of specialized transmission electron microscopes that allows for direct imaging of the atomic structure of samples. It is a powerful tool to study properties of materials on the atomic scale, such as semiconductors, metals, nanoparticles and sp2-bonded carbon (e.g., graphene, nanotubes). While …

Histology, also known as microscopic anatomy, microanatomy or histoanatomy, is the branch of biology that studies the microscopic anatomy of biological tissues. Histology is the microscopic counterpart to gross anatomy, which looks at larger structures visible without a microscope. Historically, microscopic anatomy was divided into organology, the study of organs, …

Histopathology (compound of three Greek words: ἱστός histos 'tissue', πάθος pathos 'suffering', and -λογία -logia 'study of') is the microscopic examination of tissue in order to study the manifestations of disease. Specifically, in clinical medicine, histopathology refers to the examination of a biopsy or surgical specimen by a pathologist, after …

Holography is a technique that allows a wavefront to be recorded and later reconstructed. It is best known as a method of generating three-dimensional images, and has a wide range of other uses, including data storage, microscopy, and interferometry. In principle, it is possible to make a hologram for any …

Holotomography (HT) is a laser technique to measure the three-dimensional refractive index (RI) tomogram of a microscopic sample such as biological cells and tissues. Because the RI can serve as an intrinsic imaging contrast for transparent or phase objects, measurements of RI tomograms can provide label-free quantitative imaging of microscopic …

Underwater acoustics (also known as hydroacoustics) is the study of the propagation of sound in water and the interaction of the mechanical waves that constitute sound with the water, its contents and its boundaries. The water may be in the ocean, a lake, a river or a tank. Typical frequencies …

Hyperspectral imaging collects and processes information from across the electromagnetic spectrum. The goal of hyperspectral imaging is to obtain the spectrum for each pixel in the image of a scene, with the purpose of finding objects, identifying materials, or detecting processes. There are three general types of spectral imagers. There …

Flow cytometry (FC) is a technique used to detect and measure the physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally …

X-ray scattering techniques are a family of analytical techniques which reveal information about the crystal structure, chemical composition, and physical properties of materials and thin films. These techniques are based on observing the scattered intensity of an X-ray beam hitting a sample as a function of incident and scattered angle, …

Infrared thermography (IRT), also known as thermal imaging, is a measurement and imaging technique in which a thermal camera detects infrared radiation originating from the surface of objects. This radiation has two main components: thermal emission from the object's surface, which depends on its temperature and emissivity, and reflected radiation …

Infrared spectroscopy (IR spectroscopy or vibrational spectroscopy) is the measurement of the interaction of infrared radiation with matter by absorption, emission, or reflection. It is used to study and identify chemical substances or functional groups in solid, liquid, or gaseous forms. It can be used to characterize new materials or …

An inverted microscope is a microscope with its light source and condenser on the top, above the stage pointing down, while the objectives and turret are below the stage pointing up. It was invented in 1850 by J. Lawrence Smith, a faculty member of Tulane University (then named the Medical …

In physics the magneto-optic Kerr effect (MOKE) or the surface magneto-optic Kerr effect (SMOKE) is one of the magneto-optic effects. It describes the changes to light reflected from a magnetized surface. It is used in materials science research in devices such as the Kerr microscope, to investigate the magnetization structure …

Laser Doppler velocimetry, also known as laser Doppler anemometry, is the technique of using the Doppler shift in a laser beam to measure the velocity in transparent or semi-transparent fluid flows or the linear or vibratory motion of opaque, reflecting surfaces. The measurement with laser Doppler anemometry is absolute and …

Laser Doppler imaging (LDI), also called laser Doppler perfusion imaging (LDPI), is a non-contact optical technique for mapping signals related to microvascular blood perfusion in tissue. It illuminates tissue with coherent laser light and analyzes temporal fluctuations in the backscattered light caused by scattering from moving red blood cells; these …

Inductively coupled plasma mass spectrometry (ICP-MS) is a type of mass spectrometry that uses an inductively coupled plasma to ionize the sample. It atomizes the sample and creates atomic and small polyatomic ions, which are then detected. It is known and used for its ability to detect metals and several …